Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
نویسندگان
چکیده
In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on highly conserved region within Capsid gene PPV7. The established was 1,000 times more than conventional had limit 35.6 copies. This specific no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, PPV6. Experiments testing intra interassay precision demonstrated high reproducibility. Testing newly 200 clinical samples revealed rate up to 17.5% higher that assay. could provide technical support diagnosis epidemiological investigation
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ژورنال
عنوان ژورنال: Polish Journal of Veterinary Sciences
سال: 2023
ISSN: ['2300-2557', '1505-1773']
DOI: https://doi.org/10.24425/pjvs.2021.136791